Production of a monoclonal antibody against SARS-CoV spike protein with single intrasplenic immunization of plasmid DNA.
Identifieur interne : 004713 ( Main/Exploration ); précédent : 004712; suivant : 004714Production of a monoclonal antibody against SARS-CoV spike protein with single intrasplenic immunization of plasmid DNA.
Auteurs : Xiao Fei Yu [République populaire de Chine] ; Lin Hui Liang ; Ming She ; Xiao Long Liao ; Jie Gu ; Yan Han Li ; Zhong Chao HanSource :
- Immunology letters [ 0165-2478 ] ; 2005.
Descripteurs français
- KwdFr :
- Animaux, Anticorps monoclonaux (biosynthèse), Anticorps monoclonaux (immunologie), Cellules Vero (virologie), Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires (génétique), Glycoprotéines membranaires (immunologie), Hybridomes (cytologie), Hybridomes (métabolisme), Immunisation, Lignée cellulaire tumorale, Mâle, Plasmides, Protéines de l'enveloppe virale (génétique), Protéines de l'enveloppe virale (immunologie), Rate (cytologie), Rate (immunologie), Souris, Spécificité des anticorps, Transfection, Vaccins à ADN (administration et posologie), Vaccins à ADN (génétique), Vaccins à ADN (immunologie), Virus du SRAS (immunologie).
- MESH :
- administration et posologie : Vaccins à ADN.
- biosynthèse : Anticorps monoclonaux.
- cytologie : Hybridomes, Rate.
- génétique : Glycoprotéines membranaires, Protéines de l'enveloppe virale, Vaccins à ADN.
- immunologie : Anticorps monoclonaux, Glycoprotéines membranaires, Protéines de l'enveloppe virale, Rate, Vaccins à ADN, Virus du SRAS.
- métabolisme : Hybridomes.
- virologie : Cellules Vero.
- Animaux, Glycoprotéine de spicule des coronavirus, Immunisation, Lignée cellulaire tumorale, Mâle, Plasmides, Souris, Spécificité des anticorps, Transfection.
English descriptors
- KwdEn :
- Animals, Antibodies, Monoclonal (biosynthesis), Antibodies, Monoclonal (immunology), Antibody Specificity, Cell Line, Tumor, Chlorocebus aethiops, Hybridomas (cytology), Hybridomas (metabolism), Immunization, Male, Membrane Glycoproteins (genetics), Membrane Glycoproteins (immunology), Mice, Plasmids, SARS Virus (immunology), Spike Glycoprotein, Coronavirus, Spleen (cytology), Spleen (immunology), Transfection, Vaccines, DNA (administration & dosage), Vaccines, DNA (genetics), Vaccines, DNA (immunology), Vero Cells (virology), Viral Envelope Proteins (genetics), Viral Envelope Proteins (immunology).
- MESH :
- chemical , administration & dosage : Vaccines, DNA.
- chemical , biosynthesis : Antibodies, Monoclonal.
- chemical , genetics : Membrane Glycoproteins, Vaccines, DNA, Viral Envelope Proteins.
- chemical , immunology : Antibodies, Monoclonal, Membrane Glycoproteins, Vaccines, DNA, Viral Envelope Proteins.
- cytology : Hybridomas, Spleen.
- immunology : SARS Virus, Spleen.
- metabolism : Hybridomas.
- virology : Vero Cells.
- Animals, Antibody Specificity, Cell Line, Tumor, Chlorocebus aethiops, Immunization, Male, Mice, Plasmids, Spike Glycoprotein, Coronavirus, Transfection.
Abstract
Severe acute respiratory syndrome (SARS) is a highly infectious disease caused by a novel coronavirus (SARS-CoV). Specific monoclonal antibodies (mAbs) against the SARS-CoV are vital for early diagnosis and pathological studies of SARS. Direct intrasplenic inoculation of plasmid DNA encoding antigen is an effective and fast approach to generate specific mAb when the protein antigen is difficult to prepare or dangerous in use. In this study, we selected one fragment of SARS-CoV spike protein (S1-(3)) as antigenic determinant by immunoinformatics. Single intrasplenic immunization of plasmid DNA encoding S1-(3) induced anti-spike protein antibodies. We established one hybridoma cell line secreting specific mAb and evaluated this mAb with murine leukemia virus pseudotyped with SARS-CoV spike protein (MLV/SARS-CoV). The mAb could recognize the spike protein on the MLV/SARS-CoV-infected Vero E6 cells albeit with no neutralizing effect on the infectivity of the pseudotype virus. Our results show that a single-shot intrasplenic DNA immunization is efficient for the production of specific mAb against SARS spike protein, and a linear epitope of the spike protein is recognized in this study.
DOI: 10.1016/j.imlet.2005.03.015
PubMed: 15893826
Affiliations:
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Le document en format XML
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uniqKey="Yu X" first="Xiao Fei" last="Yu">Xiao Fei Yu</name><affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Experimental Hematology, Institute of Hematology, Chinese Academy of Medical Sciences & Peking Union Medical College, 288 Nanjing Road, Tianjin 300020, PR China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Experimental Hematology, Institute of Hematology, Chinese Academy of Medical Sciences & Peking Union Medical College, 288 Nanjing Road, Tianjin 300020</wicri:regionArea><placeName><settlement type="city">Tianjin</settlement></placeName></affiliation></author><author><name sortKey="Liang, Lin Hui" sort="Liang, Lin Hui" uniqKey="Liang L" first="Lin Hui" last="Liang">Lin Hui Liang</name></author><author><name sortKey="She, Ming" sort="She, Ming" uniqKey="She M" first="Ming" last="She">Ming She</name></author><author><name sortKey="Liao, Xiao Long" sort="Liao, Xiao Long" uniqKey="Liao X" first="Xiao Long" last="Liao">Xiao Long Liao</name></author><author><name sortKey="Gu, Jie" sort="Gu, Jie" uniqKey="Gu J" first="Jie" last="Gu">Jie Gu</name></author><author><name sortKey="Li, Yan Han" sort="Li, Yan Han" uniqKey="Li Y" first="Yan Han" last="Li">Yan Han Li</name></author><author><name sortKey="Han, Zhong Chao" sort="Han, Zhong Chao" uniqKey="Han Z" first="Zhong Chao" last="Han">Zhong Chao Han</name></author></analytic><series><title level="j">Immunology letters</title><idno type="ISSN">0165-2478</idno><imprint><date when="2005" type="published">2005</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Antibodies, Monoclonal (biosynthesis)</term><term>Antibodies, Monoclonal (immunology)</term><term>Antibody Specificity</term><term>Cell Line, Tumor</term><term>Chlorocebus aethiops</term><term>Hybridomas (cytology)</term><term>Hybridomas (metabolism)</term><term>Immunization</term><term>Male</term><term>Membrane Glycoproteins (genetics)</term><term>Membrane Glycoproteins (immunology)</term><term>Mice</term><term>Plasmids</term><term>SARS Virus (immunology)</term><term>Spike Glycoprotein, Coronavirus</term><term>Spleen (cytology)</term><term>Spleen (immunology)</term><term>Transfection</term><term>Vaccines, DNA (administration & dosage)</term><term>Vaccines, DNA (genetics)</term><term>Vaccines, DNA (immunology)</term><term>Vero Cells (virology)</term><term>Viral Envelope Proteins (genetics)</term><term>Viral Envelope Proteins (immunology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Anticorps monoclonaux (biosynthèse)</term><term>Anticorps monoclonaux (immunologie)</term><term>Cellules Vero (virologie)</term><term>Glycoprotéine de spicule des coronavirus</term><term>Glycoprotéines membranaires (génétique)</term><term>Glycoprotéines membranaires (immunologie)</term><term>Hybridomes (cytologie)</term><term>Hybridomes (métabolisme)</term><term>Immunisation</term><term>Lignée cellulaire tumorale</term><term>Mâle</term><term>Plasmides</term><term>Protéines de l'enveloppe virale (génétique)</term><term>Protéines de l'enveloppe virale (immunologie)</term><term>Rate (cytologie)</term><term>Rate (immunologie)</term><term>Souris</term><term>Spécificité des anticorps</term><term>Transfection</term><term>Vaccins à ADN (administration et posologie)</term><term>Vaccins à ADN (génétique)</term><term>Vaccins à ADN (immunologie)</term><term>Virus du SRAS (immunologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="administration & dosage" xml:lang="en"><term>Vaccines, DNA</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Antibodies, Monoclonal</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Membrane Glycoproteins</term><term>Vaccines, DNA</term><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Antibodies, Monoclonal</term><term>Membrane Glycoproteins</term><term>Vaccines, DNA</term><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" qualifier="administration et posologie" xml:lang="fr"><term>Vaccins à ADN</term></keywords><keywords scheme="MESH" qualifier="biosynthèse" xml:lang="fr"><term>Anticorps monoclonaux</term></keywords><keywords scheme="MESH" qualifier="cytologie" xml:lang="fr"><term>Hybridomes</term><term>Rate</term></keywords><keywords scheme="MESH" qualifier="cytology" xml:lang="en"><term>Hybridomas</term><term>Spleen</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Glycoprotéines membranaires</term><term>Protéines de l'enveloppe virale</term><term>Vaccins à ADN</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Anticorps monoclonaux</term><term>Glycoprotéines membranaires</term><term>Protéines de l'enveloppe virale</term><term>Rate</term><term>Vaccins à ADN</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>SARS Virus</term><term>Spleen</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Hybridomas</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Hybridomes</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Cellules Vero</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Vero Cells</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Antibody Specificity</term><term>Cell Line, Tumor</term><term>Chlorocebus aethiops</term><term>Immunization</term><term>Male</term><term>Mice</term><term>Plasmids</term><term>Spike Glycoprotein, Coronavirus</term><term>Transfection</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Glycoprotéine de spicule des coronavirus</term><term>Immunisation</term><term>Lignée cellulaire tumorale</term><term>Mâle</term><term>Plasmides</term><term>Souris</term><term>Spécificité des anticorps</term><term>Transfection</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Severe acute respiratory syndrome (SARS) is a highly infectious disease caused by a novel coronavirus (SARS-CoV). Specific monoclonal antibodies (mAbs) against the SARS-CoV are vital for early diagnosis and pathological studies of SARS. Direct intrasplenic inoculation of plasmid DNA encoding antigen is an effective and fast approach to generate specific mAb when the protein antigen is difficult to prepare or dangerous in use. In this study, we selected one fragment of SARS-CoV spike protein (S1-(3)) as antigenic determinant by immunoinformatics. Single intrasplenic immunization of plasmid DNA encoding S1-(3) induced anti-spike protein antibodies. We established one hybridoma cell line secreting specific mAb and evaluated this mAb with murine leukemia virus pseudotyped with SARS-CoV spike protein (MLV/SARS-CoV). The mAb could recognize the spike protein on the MLV/SARS-CoV-infected Vero E6 cells albeit with no neutralizing effect on the infectivity of the pseudotype virus. Our results show that a single-shot intrasplenic DNA immunization is efficient for the production of specific mAb against SARS spike protein, and a linear epitope of the spike protein is recognized in this study.</div></front></TEI><affiliations><list><country><li>République populaire de Chine</li></country><settlement><li>Tianjin</li></settlement></list><tree><noCountry><name sortKey="Gu, Jie" sort="Gu, Jie" uniqKey="Gu J" first="Jie" last="Gu">Jie Gu</name><name sortKey="Han, Zhong Chao" sort="Han, Zhong Chao" uniqKey="Han Z" first="Zhong Chao" last="Han">Zhong Chao Han</name><name sortKey="Li, Yan Han" sort="Li, Yan Han" uniqKey="Li Y" first="Yan Han" last="Li">Yan Han Li</name><name sortKey="Liang, Lin Hui" sort="Liang, Lin Hui" uniqKey="Liang L" first="Lin Hui" last="Liang">Lin Hui Liang</name><name sortKey="Liao, Xiao Long" sort="Liao, Xiao Long" uniqKey="Liao X" first="Xiao Long" last="Liao">Xiao Long Liao</name><name sortKey="She, Ming" sort="She, Ming" uniqKey="She M" first="Ming" last="She">Ming She</name></noCountry><country name="République populaire de Chine"><noRegion><name sortKey="Yu, Xiao Fei" sort="Yu, Xiao Fei" uniqKey="Yu X" first="Xiao Fei" last="Yu">Xiao Fei Yu</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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